Figure 7.

CD28 costimulation enhances cytokine release from peripheral blood mononuclear cells (PBMCs) upon antigenic recall stimulation with purified protein derivative or tetanus and diphtheria toxoid in vitro. (A) Interferon γ (IFNγ) and (B) interleukin-5 concentrations in supernatants of experiments are first shown in Figure 4, i.e., under Th0 (left), T helper (Th1) (middle), or Th2 (right) culture conditions. Please note that for human T cells Th1 conditions did not comprise addition of an anti-IFNγ antibody as was the case for mouse Th1 conditions. Graphs show data from 19 donors assayed individually and tested with a one-tailed paired t-test (*p < 0.05, **p < 0.005).