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. 2018 May 22;8:8020. doi: 10.1038/s41598-018-26407-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell viability and proliferation within 3D constructs (A) Representative images of Live/Dead staining of 3D bioprinted constructs at Day1 and Day14 of culture (Scale bar: 200 μm); (B) Live/Dead cell counting and quantitative analysis at Day1, 3, 7 and 14 of culture(^**p < 0.01); (C) Representative images of Ki-67 staining of 3D bioprinted constructs at Day1 and Day14 of culture (Scale bar: 50 μm); (D) Ki-67 positive cell counting and quantitative analysis at Day1, 3, 7 and 14 of culture (a: ^**p < 0.01 compared with B-2, B-3 and B-4 at Day14; b: ^**p < 0.01 compared with B-3 and B-4 at Day14).