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. 2018 May 22;8:8020. doi: 10.1038/s41598-018-26407-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Images of cell aggregation within 3D constructs (A) Images of glandular cell clusters discovered in the B-2 during 28 days of culture (Scale bar: 50 μm); (B) ESC clusters formation in constructs at Day14 (Scale bar: 50 μm) and Day28 (Scale bar: 200 μm) of culture (White arrows: ESC clusters).