Fig. 2. PF promoted the degradation of β-catenin.

a HEK293T cells were incubated with PF (1, 2 and 4 μM) in the presence of LiCl (20 mM). After 16 h, luciferase activity was determined. Data represent the means ± SD (n = 3), *P < 0.05, **P < 0.01, compared with LiCl group, ^##P < 0.01, compared with untreated group. b The protein levels of β-catenin and target proteins were observed after treated with different concentrations of PF and LiCl (20 mM) for 16 h. c, d HEK293T cells were treated with 4 μM PF for 12 h, followed with or without 10 μM MG132 for additional 8 h. β-catenin was immunoprecipitated with a β-catenin antibody. Input and immunoprecipitated fractions were analysed by immunoblotting