Figure 3.

Cypripedin attenuated in vitro tumourigenesis and spheroid-based cell migration. (A) H460 cells were mixed with 4% Matrigel and cultured onto Matrigel coated-cell culture plate in the presence or absence of cypripedin (20 µM). After 10 d, spheroid was immunostained for actin (red) and DNA (blue). The data are presented as a mean of spheroid diameter ± SEM (n = 25). *p < 0.05 compared with control cells. Scale bar is 20 µm. (B) Spheroids were generated under detached condition as described in Method, seeded onto cell culture plate and treated with or without cypripedin (20 µM). Images were captured at d0 and d3 with 20x and 40x magnification, and cell migration was analyzed from the migrating distance (between red line and black line). The data are presented as a mean of migrating distance ± SEM (n = 15). *p < 0.05 compared with control cells. Scale bar is 100 µm.