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. 2018 May 16;9:935. doi: 10.3389/fmicb.2018.00935

FIGURE 2.

FIGURE 2

Uptake and intracellular replication of B. pseudomallei E8 wild type, ΔgvmR mutant and complemented mutant ΔgvmR::gvmR in (A) RAW 264.7 macrophages and (B) BALB/c-BMM macrophages. The bacteria were grown on LB agar supplemented with 5% sheep blood for 16 h at 37°C and diluted in PBS for the infection. RAW264.7 macrophages and BALB/c-BMM macrophages were infected at an MOI of 1 and 2, respectively, and intracellular CFUs were determined using the antibiotic protection assay followed by plating of infected cells on LB agar. 0 h indicates bacterial uptake and intracellular bacterial survival and replication was quantified at 6 and 24 h postinfection. Shown are mean values of three independent experiments performed in triplicate. Error bars indicate standard error of the mean (SEM). Statistical analyses were performed using one-way ANOVA with Bonferroni multiple comparisons posttest (∗∗p < 0.01; ∗∗∗p < 0.001).