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. 2018 May 16;9:935. doi: 10.3389/fmicb.2018.00935

FIGURE 4.

FIGURE 4

Growth (A), biofilm formation (B), and motility (C,D) of B. pseudomallei E8 wild type, ΔgvmR mutant and complemented mutant ΔgvmR::gvmR. (A) Bacteria were grown in M9 minimal medium at 37°C and 140 rpm for 96 h. Shown are mean values of two independent experiments. Error bars indicate standard error of the mean (SEM). black square – wild type, black triangle – ΔgvmR, black diamond – complemented strain ΔgvmR::gvmR. (B) Biofilm formation was quantified 24 and 48 h after static incubation in LB broth at 37°C. Crystal violet retention of the biofilm mass was measured at 540 nm after methanol elution of the dye. Data represent mean values of three experiments. Error bars indicate standard error of the mean (SEM). Statistical analyses were performed by using one-way ANOVA with Bonferroni multiple comparisons posttest (∗∗∗p < 0.001). (C) Swimming motility of the indicated strains in LB agar (0.3%) and (D) swarming motility in LB agar (0.6%). Swimming and swarming motility was quantified 24 and 48 h after incubation at 37°C by measuring the diameters of the circular bacterial migration from the point of inoculation. Shown are mean values of three independent experiments. Error bars indicate standard error of the mean (SEM). Statistical analyses were performed using one-way ANOVA with Bonferroni multiple comparisons posttest (∗∗∗p < 0.001).