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. 2018 May 22;9:2023. doi: 10.1038/s41467-018-04469-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Localization of IFT88 revealing a distal appendage matrix (DAM). a Three representative axial-view dSTORM images of IFT88 reveal a broad angular distribution without a clear nine-fold symmetric pattern. b Two representative sets of two-color images showing IFT88 spanning a longitudinal range relative to the location of SCLT1 (marked with a dashed line). The solid line in c depicts the distribution in the lower panel in b, whereas the dotted line illustrates normalized distributions averaged over nine data pairs. d, e 3D STORM imaging of IFT88 showing the tapered distribution of IFT88 that is wider at the distal end of the DAP region. f Three-dimensional two-color super-resolution image revealing IFT88 proteins in the gaps between neighboring SCLT1 regions, as shown in the lower image (derived from the upper-right image) in which nine SCLT1 puncta are numbered accordingly. g The region between adjacent DABs is designated as the DAM. h Model showing the localization of FBF1 and IFT88 at the DAM. i Two-color imaging of IFT88-FBF1 shows the angular coincidence between IFT88 and FBF1 at the DAM. j Histogram of angular spacing between IFT88 and FBF1. k, l The radial distribution of IFT88 was reduced in FBF1^−/− cells. m Model showing a functional role of FBF1 in maintaining the DAM spatial arrangement. Bars = 200 nm for all, except f (100 nm)