Fig. 6.

DAB components mediating the structural arrangement of the DAM. a, b Axial view dSTORM images reveal the distribution of a CEP164 and b FBF1 at the mother centrioles upon CRISPR/Cas9 FBF1 knockout and CEP164 siRNA silencing, respectively. It is noteworthy that the FBF1 ring formed upon CEP164 knockdown becomes smaller than that of controlled cells (b, right panel). c, d Statistical analysis of the radial distribution of CEP164 and FBF1 upon perturbation. The size of CEP164 in FBF1^−/− cells is comparable to that in WT cells c, whereas the size of FBF1 is reduced upon CEP164 knockdown d. e Model showing that the arrangement of FBF1 is no longer maintained at the outer region of the DAM in the absence of CEP164. f Three-dimensional model based on all axial and lateral super-resolution images illustrating the molecular architecture on the framework of DABs, DAM, axoneme, and ciliary membrane. g Close-up view of the DAP region, suggesting that FBF1 potentially interfaces the ciliary membrane and the DAM. Bars = 200 nm