Figure 2.

Carboxyfluorescein succinimidyl ester (CFSE)-labeling marks Candida glabrata yeast division. (A) Differential interference contrast and fluorescence microscopy image of CFSE-labeled C. glabrata that have undergone division and stained with calcofluor white. Parental cells exhibit full CFSE labeling, while daughter cells have diluted CFSE labeling leading to dimmer fluorescence signal. Scale bar represents 5 µm. (B) Scatter plot of Con A-AF647 and CFSE fluorescence. CFSE-bright parental yeast population in upper right quadrant gate. Percent (scatter plot inset) of undivided CFSE-bright population was determined over time indicated. (C) Correlation of percent CFSE-bright population to OD₆₀₀ nm of proliferating yeast. Data represent a minimum of three independent triplicate experiments.