Fig. 2. Hepatic EZH2 and H3K27me3 in liver failure mice.

Production of EZH2 and H3K27me3 in Kupffer cells from LPS/D-GalN-induced liver failure mice was measured at the different time points, using western blot (a) and RT-PCR (b) (n = 5 per time point). The bars represent bands density ratios of EZH2/GAPDH or H3K27me3/H3. The data are shown as the mean ± SEM. *, P < 0.05 vs. EZH2 at 0 h; ^#, P < 0.05 vs. H3K27me3 at 0 h. Two hours after intraperitoneal injection of PBS or LPS/D-GalN, mice in both groups were sacrificed and the liver tissues were collected. Representative images of immunofluorescence analysis showed co-staining of EZH2 (green) and F4/80 (red) (c), as well as H3K27me3 and F4/80 (d). Percentage analysis of EZH2⁺ F4/80⁺/F4/80⁺ cells and H3K27me3⁺ F4/80⁺/F4/80⁺ cells was performed. *, P < 0.05