Fig. 4. Proinflammatory cytokines production following addition of GSK126.

a Hepatic Tnf, Il-6 and Il-1β mRNA levels in the liver from the LPS/D-GalN-induced liver failure mice treated with or without GSK126 (n = 8 for each treatment). b Serum TNF production in LPS/D-GalN-induced liver failure mice treated with or without GSK126 (n = 8 for each treatment). c PBMC from human healthy volunteers were stimulated either GSK126 or vehicle (DMSO) 1 h prior to exposure of LPS (1 μg/ml) for the indicated times. TNF mRNA was measured using quantitative RT-PCR. *, P < 0.05; **, P < 0.01; ***, P < 0.001