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. 2018 May 22;8:8001. doi: 10.1038/s41598-018-26123-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR/Cas9 Targeting of EDNRB in isogenic iPS cells. (A) Structure of the EDNRB locus showing localization of two guides that were used to ablate all 4 isoforms. (B) Genotyping of isogenic clones after CRISPR/Cas9 editing showing EDNRB^+/+, EDNRB^+/− and EDNRB^−/− clones. (C) FACS analysis for CD34⁺ generated from Cas9 edited disomic and trisomic isogenic cells. (D) Quantification of B cell production in differentiated cells, presented as number of B cells generated per input CD34⁺ cell (p-value ≤ 0.15, between disomic KO and disomic WT/Het). G1 and G2 refer to two guides that were used for EDNRB editing.