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. 2018 May 22;8:8000. doi: 10.1038/s41598-018-26255-1

Figure 5.

Figure 5

Cytotoxicity of Htt C-terminal fragment is partially attenuated upon the expression of a Htt-interacting domain in kalirin. (a) STHdh7Q/7Q striatal cells were transfected with plasmid DNAs expressing FLAG-tagged 18QHtt1-587, 100QHtt1-587, or Htt2568-3144 for 14 hours and processed for the MTT conversion assay. For each transfection condition, 3 independent transfections were performed. (b) STHdh7Q/7Q striatal cells were transfected with the Htt2568-3144 expressing plasmid with or without plasmids expressing 6xHis-tagged Klrn23-684 or Klrn674-1272 or Klrn1269-1654 or Rac1G12V for 12 to 14 hours. Appropriate amounts of empty vector DNAs were supplemented to make the amount of plasmid DNAs equal for each transfection condition. Two independent transfections were conducted for each condition. In both (a) and (b), empty pcDNA3 vector was used as the mock transfection control. Quantification of MTT conversion for each transfection condition was conducted in duplicate. The average of MTT conversion from different transfections for each condition was calculated. Data were represented as the percentage of MTT conversion in cells transfected with empty vector. Statistical significance was determined by two-tailed Student t-test.