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. 2018 May 22;4:12. doi: 10.1038/s41522-018-0055-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Analysis of the quantitative composition of the multispecies oral biofilms. Quantification was performed used CFU counting for each species per biofilm disc as described in the Methods. As indicated, control biofilms did not include an A. actinomycetemcomitans strain (Mix A), whereas experimental biofilms either contained strain D7S (mix B) or its hns mutant (Mix C). The data are expressed as the bacterial mean counts ± SD from 10 biological replicates. ANOVA test: ****p < 0.0001