Fig. 7. The effects of Gm2199 on the activation and the expression of ERK1/2 in damaged hepatocytes.

Representative western blot analysis results for p-ERK1/2 and T-ERK1/2 protein expression from Control, CCl₄, CCl₄ + pEGFP-N1-Gm2199, and CCl₄ + pEGFP-N1 vector-treated AML12 (a) and NCTC1469 (b). QRT-PCR analysis results for ERK1 and ERK2 mRNA expression from Control, CCl₄, CCl₄ + pEGFP-N1-Gm2199, and CCl₄ + pEGFP-N1 vector-treated AML12 (c) and NCTC1469 (d). The experiment data were converted to relative values over the control group and were expressed as mean ± SEM (n = 3 for each group). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. CCl₄