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. 2018 May 22;9(6):608. doi: 10.1038/s41419-018-0644-4

Fig. 5. Exogenous overexpression of SNAIL significantly rescued the antitumor effect of TRIM50.

Fig. 5

a, b BEL7402 cells (a) and HUH7 cells (b) were transfected with TRIM50 plasmid and SNAIL plasmid, and the expression of TRIM50 and SNAIL were detected by western blot. c BEL7402 and HUH7 cells were transfected with TRIM50 plasmid or SNAIL plasmid, and transwell invasive assay was performed to detect the invasive capability of these transfected HCC cells. d BEL7402 cells were transfected with TIRM50 expression plasmid or SNAIL expression plasmid, and the cells were transferred to six-well plates at the density of 1000 cells per well for colony formation assay. The colonies were stained after 14 days, and the number of colonies was counted and statistically analyzed. **P < 0.01 and ***P < 0.001 for statistical analysis of the indicated groups