Fig. 6. Antitumor effect of TRIM50 was verified in xenografted tumor model.

BEL7402 cells (10⁷ cells) were subcutaneously injected to both flanks of the nude mice. When visible tumor appeared, TRIM50 expression plasmid was injected to the formed tumor in the left flank and mock control plasmid was injected to the tumor in the right flank every other day before the mice were sacrificed on day 28. a The growth curves of tumors with TRIM50 or mock plasmid transfection were analyzed every other day before the mice were sacrificed. b The formed tumors with TRIM50 or mock plasmid transfection were isolated and compared. c Images presented were the representative mice with subcutaneous xenograft tumor. d, e The volume (d) and weight (e) of the formed tumors transfected with TRIM50 plasmid or mock plasmid were analyzed and compared. f mRNA levels of TRIM50 in TRIM50 or mock control transfected tumors were analyzed by qRT-PCR. g Western blot assay was performed to detect the protein levels of TRIM50 and SNAIL in the formed tumors. h Immunohistochemical staining was used to detect the level of TRIM50, E-cadherin, β-catenin, N-cadherin, and SNAIL in formed tumors. *P < 0.05, **P < 0.01, and ***P < 0.001 for statistical analysis of the indicated groups