Fig. 1.

Knockout of Intu from kidney proximal tubular cells aggravates renal IRI in mice. a Breeding protocol for generating PT-Intu-KO mouse model. b Induction of Intu in mouse kidneys by 35 min of bilateral renal ischemia with 6 or 48 h of reperfusion. c Intu expression at renal IRI (48 h) was abolished in PT-Intu-KO mice as compared to PT-Intu-WT mice (n = 3). d PT-Intu-KO mice and PT-Intu-WT mice had similarly low levels of BUN and SCr under control condition (n = 9), but renal IRI induced higher BUN and SCr in PT-Intu-KO mice (n = 9). e Renal IRI induced more severe renal tubular damage in PT-Intu-KO mice than in PT-Intu-WT mice as shown by representative histology and quantification of the percentage of damaged tubules (n = 6). Quantitative data are mean ± s.d. (error bars). Paired or grouped t test was used. *p < 0.05, **p < 0.01. Scale bar: 100 µm. AU arbitrary unit