Fig. 2.

Suppression of Intu increases cell death during renal IRI injury in vivo and metabolic stress in vitro. a TUNEL assay showing more death cells (red) in proximal tubules (green by LTA staining) of PT-Intu-KO kidney tissues following renal IRI than PT-Intu-WT (n = 8). Please note that LTA staining pattern was disrupted due to injury. The asterisk indicates a likely non-PT tubule with no LTA staining and different morphology. b, c Establishment of stable Intu-knockdown BUMPT cells by transfecting Intu shRNAs (shIntu) confirmed by immunoblot, qRT PCR, and immunostaining. d Representative cell and nuclear images showing more cell death in Intu-knockdown cells after azide or CCCP treatment. Two knockdown clones targeting different sites were presented. e Quantification of cell death in Intu-knockdown cells and control shRNA-transfected cells after azide or CCCP treatment. Quantitative data are mean ± s.d. (error bars). Paired or grouped t test was used. *p < 0.05, **p < 0.01, ***p < 0.001. Cell nuclei were stained with DAPI. Scale bar (µm): 20 (a, d), 10 (c)