Fig. 3.

Inducible Intu expression suppresses cell death. a Inducible Intu expression in RPTC cells. Three inducible cell clones (D-REx Intu3, 5, 10) were treated with doxycycline to induce Intu expression shown by immunoblotting in comparison to three empty vector controls (D-REx CTL1, 2, 3). b Less cell death was observed in Intu-expressing cells after azide treatment. c Resistance of Intu-expressing cells to cisplatin treatment. d Immunoblot analysis of Caspase-3 and Parp cleavage to confirm less apoptosis in Intu-expressing cells after azide or cisplatin treatment. Quantitative data are mean ± s.d. (error bars). Grouped t test was used.*p < 0.05, ***p < 0.001. Scale bar: 20 µm