Fig. 3.

Microglia trogocytosis of presynaptic boutons and axons. Representative FIB-SEM image sequences of a complete presynaptic bouton inclusion (dark purple), as identified by its 40 nm vesicles content, inside microglia (red), b partial inclusion containing axonal material (clear blue) inside a microglia. c Quantification of microglial partial and complete inclusions (n = 37 inclusions from 8 cells, 4 animals). d Distribution of the volume of microglial inclusions. e Putative sequence of events leading to presynaptic bouton or axon material digestion by microglia, represented by a schematic and a collection of three examples for each step (gray: undetermined origin, yellow: lysosomes). Scale bars: 200 nm