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. 2018 May 22;4:24. doi: 10.1038/s41421-018-0023-4

Fig. 3. Localization of selected enzymes in E. falciformis merozoites developing within host cells.

Fig. 3

a Schematics of the expression cassette used to express a protein of interest in E. falciformis (EfPOI) under the control of EfHIS4 promoter and 3’UTR. b Immunofluorescence images of transgenic merozoites expressing EfG3PAT2-HA, EfCDS2-HA, EfPIS-HA, EfCDS1-HA, and EfPSD2-HA. Plasmid constructs harboring the indicated ORFs were transfected into sporozoites followed by infection of HFF cells, and immunostaining with anti-HA antibody and E. tenella antiserum cross-reactive to E. falciformis (40–42 h post infection). Non-transfected control parasites exhibited no apparent staining with anti-HA antibody. Scale bars: 2 μm