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. 2018 May 22;4:24. doi: 10.1038/s41421-018-0023-4

Fig. 6. EfPTS can produce tachyzoite-specific PtdThr species in the PTS knockout strain of T. gondii.

Fig. 6

a, b MS analysis of PtdThr isolated from the sporozoites of E. falciformis or from the tachyzoites of T. gondii. Lipid species were identified by their product spectra in the negative ionization mode. Note that the dominant PtdThr species in Eimeria harbor C36:1/C36:2 acyl chains in contrast to C40:5 in Toxoplasma. c, d MS/MS spectra of the major PtdThr peaks (m/z 802.6 for E. falciformis and m/z 850.6 for T. gondii). Acyl chains were identified as 18:0/18:1 (C36:1) and 20:1/20:4 (C40:5). e MS analysis of lipids isolated from the parental, Δtgpts and Δtgpts/EfPTS strains of T. gondii. The data show a major (40:5) and several minor (40:6, 38:4, 38:5, and 36:4) PtdThr species in the parental strain, which are all absent in the Δtgpts mutant. The loss of all indicated lipid species is restored by expression of EfPTS in the Δtgpts/EfPTS strain