Fig. 5.

Growth on xylose facilitated by the xylose regulon. a Design of the genes placed under the semi-synthetic xylose regulon and its mode of activation. Apart from GAL3-Syn4.1 placed under GAL3p and GAL1p promoters, other metabolic genes XYLA*3, XKS1, TAL1, and xylose transporter GAL2-2.1 were expressed under GAL1p and GAL10p promoters. b Design of the metabolic control strain (XYL-CONS) built by placing the genes XYLA*3, XKS1, TAL1, and GAL2-2.1 under strong TEF1p and TPI1p promoters. c Growth (bold lines) and xylose consumption profile (dotted lines) of the engineered strain, XYL-REG, and metabolic control, XYL-CONS under aerobic conditions. Each data point represents average of three individual replicates ± sd