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Western Blot of Ileal Brush Border Proteins from Wild-Type, Heterozygous and Cystic Fibrosis Mice. An increase in IBAT protein concentration was observed in all examined brush border membrane vesicle preparations of the terminal ileum in knock-out mice. IBAT content approximately doubled in heterozygous knock-out mice and quadrupled in homozygous mutants compared to wild-type controls.
