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Analytical sensitivity of the cobas EGFR assay for NHP spiked with five different levels of mutant cfDNA. Copy numbers and frequencies of mutant alleles are provided in Supplementary Table S3. Dots in the figure represent negative measurement (ND) or the SQI value of the positive measurement for (a) p.E746_A750del (exon 19 deletion), (b) p.L858R, (c) p.T790M, and (d) p.A767_V769dup (exon 20 insertion) mutations.
