FIG 4.

Induction of Ng-MscS containing a gain-of-function mutation inhibits growth of E. coli. Growth curves of E. coli strain MJF465 expressing wild-type Ng-MscS or Ec-MscS or channels containing gain-of-function mutations in the hydrophobic gas vapor lock were generated with or without induction with IPTG at 2 h postinoculation. The graphs represent the means and standard deviations of data from three independent experiments. (A) Ng-MscS; (B) Ng-MscS(L105A); (C) Ec-MscS; (D) Ec-MscS(L09S). Significant differences for each time point between induced and uninduced conditions were identified by Student's two-tailed unpaired t test (GraphPad Prism). ***, P < 0.001.