FIG 5.

Differential response of Mtb^A and Mtb^P to oxidative stress. (A) Normalized transcript abundances of differentially expressed genes involved in redox stress mitigation in Mtb^A and Mtb^P 10 days postinfection. (B) Cytosolic redox potential (E_MSH) under basal conditions and in response to 10 mM cumene hydroperoxide (CHP) or 40 mM dithiotreitol (DTT) in Mtb^A and Mtb^P 10 days postinfection. *, P < 0.05; ***, P < 0.001. (C) Growth assay of Mtb^A and Mtb^P 10 days postinfection in the presence of different concentrations of oxidizing agents (0.1 and 0.01 mM CHP and 0.01 and 0.02 mM plumbagin [PB]) in 7H10 OADC or OADS plates. (D) Growth kinetics of ΔbfrAB and wild-type H37Rv in adipocytes (A) and preadipocytes (P) as assessed by CFU counts. Data are means ± standard deviations for three wells (representative of three experiments).