FIG 1.

TNF antagonism induces increased Treg levels in H. capsulatum-infected mice. Foxp3-GFP mice were infected with 2 × 10⁶ H. capsulatum yeasts i.n. and treated with anti-TNF or isotype control antibody. Lungs were harvested at day 4 p.i. and assessed for Tregs via flow cytometry. (A) Representative density plots of CD4⁺ CD25⁺ Foxp3⁺ T cells. Cells demarcated by the diamonds represent CD4⁺ CD25⁺ Foxp3⁺ Tregs. (B) Percentages of CD4⁺ CD25⁺ Foxp3⁺ T cells. (C) Cell number proportions of CD3⁺ CD4⁺ CD25⁺ Foxp3⁺ Tregs to total CD3⁺ CD4⁺ T lymphocytes. (D) Representative histograms of Foxp3 MFI. (E) MFI of Foxp3 gated on CD4⁺ CD25⁺ cells. (F) CD4⁺ CD25⁺ Foxp3⁺ Tregs were sorted from day 4 p.i. lungs, and RNA was extracted. Helios gene expression was assessed by qRT-PCR. The data represent means and standard errors of the mean (SEM) (n = 4 to 8 mice/group; representative of the results of at least two experiments). *, P < 0.05; **, P < 0.01; #, P < 0.001.