FIG 2.
Production of recombinant anti-PTx antibodies. (A) Purity and size of purified IgG antibodies. Protein (3 μg) samples were run under reducing SDS-PAGE conditions, and the sizes of molecular size (MW) markers are indicated. (B) Melting temperature profiles of the purified recombinant antibodies, determined by differential scanning fluorimetry. The curves shown are the averages for four replicates. The melting temperature (Tm) for each antibody was determined from the minimum of the transition temperatures in the derivative data (−dF/dT) and is shown in Table 1.