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Antibody D8 suppresses PTx-induced leukocytosis in mice. Mice (groups of 4) were administered PBS, 2 μg PTx, or 2 μg PTx plus 20 μg antibody by subcutaneous injection in a 100-μl volume. Four days later, blood was harvested by cardiac puncture, and CD45+ leukocytes were measured by flow cytometry. Groups were compared using ANOVA and Tukey's test (α = 0.05; **, P < 0.01; ****, P < 0.0001).
