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. 2018 May 22;86(6):e00123-18. doi: 10.1128/IAI.00123-18

FIG 2.

FIG 2

Growth curves for R. parkeri wild-type, sca2::tn, and rickA::tn strains in Vero (A) and ISE6 (B) cells. Data are fold change compared to initial input. Collection times consisted of 30 mpi and 2, 8, 24, 48, 72, 96, and 120 hpi. There was a lack of statistical significance at all time points for R. parkeri sca2::tn and rickA::tn strains compared to wild-type R. parkeri across both cell types. Data are representative of three replicates per experiment and two independent experiments. A Kruskal-Wallis test with Dunn's post hoc analysis was completed in GraphPad Prism software. A P value of <0.05 was considered significant. Western blots showing expression or lack of expression of Sca2 (C) and RickA (E) in R. parkeri sca2::tn and rickA::tn. Densitometric analysis of Sca2 (D) and RickA (F) expression in respective mutant strains was completed by normalizing expression of each protein against expression of β-actin in Vero cells.