Presence of R. parkeri wild type, sca2::tn, and rickA::tn in the hemolymph of exposed A. maculatum. Mean rickettsial load was quantified at 12 h (left), 3 days (middle), and 7 days (right) postexposure. Statistical analysis consisted of a one-way ANOVA followed by Tukey's post hoc analysis, with a P value of <0.05 considered significant (denoted by an asterisk). Error bars represent the SEM. The mean is represented by the bar between the SEM. NS indicates nonsignificant data sets compared to wild-type data. Data are representative of two replicates per experiment and two independent experiments.