TABLE 1.
List of primers and probes used for qPCR
| Primer set or probea | Sequenceb (5′−3′) | Partial gene amplified | Reference or source |
|---|---|---|---|
| RpompB129FJJ | CAAATGTTGCAGTTCCTCTAAATG | R. parkeri ompB | 38 |
| RpompB224RJJ | AAAACAAACCGTTAAAACTACCG | ||
| RparompB | FAM-TTTG+A+G+C+A+G+CA-IABkFQ | ||
| AmacMIF.18F | CCAGGGCCTTCTCGATGT | A. maculatum mif | 28 |
| AmacMIF.99R | CCATGCGCAATTGCAAACC | ||
| AmacMIF.63 | HEX-TGTTCTCCTTTGGACTCAGGCAGC | ||
| Vero b-actin.61F | TGAAGTGTGACGTGGACATCCATA | Vero β-actin | 29 |
| Vero b-actin.170 R | GGCAGTAATCTCCTTCTGCATCCT | ||
| Vero b-actin.116 | TGGCACCACCATGTACCCTGGCATTGCT | ||
| ISE6_calFW | AGCAGGGAACTTTCAAGCTG | I. scapularis crt | This paper |
| ISE6_calREV | AGAAAGGCTCGAACTTGGTG | ||
| ISE6_cal.67 | HEX-AGACCTCTGAAGATGCCCGCTTT |
a
A plus sign denotes the use of a locked nucleic acid.
b
FAM, 6-carboxyfluorescein; IABkFQ, Iowa Black dark quencher; HEX, hexachlorofluorescein.