FIG 2.

BcfA activates murine bone marrow-derived dendritic cells. Murine BMDCs derived from C3H/HeJ mice were stimulated for 20 to 24 h with various doses of BcfA. The supernatant was collected, and the cells were harvested for analysis of costimulatory molecule expression. (A) Expression of CD40, CD80, and CD86 following BcfA stimulation. Hatched line, medium alone; dark line, BcfA stimulation. One representative well stimulated with 10 μg/ml of BcfA is shown. (B) Supernatant was tested by sandwich ELISA for production of cytokines IL-6, TNF-α, and IL-12/23 common chain. Bars represent the average values for 3 mice tested in triplicate under each condition. One representative experiment of 3 independent experiments is shown. *, P < 0.05; **, P < 0.005; ***, P < 0.001.