Reduced Th2 cytokine production from spleen cells of mice immunized with BcfA-adjuvanted vaccines. Spleens from immunized mice harvested at day 3 postinoculation were stimulated for 7 days with FHA. Supernatant was tested by sandwich ELISA for the presence of IFN-γ (A), IL-17 (B), and IL-5 (C). One representative experiment of two, with 4 or 5 mice per group, is shown. *, P < 0.05; **, P < 0.005; ***, P < 0.001.