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. Author manuscript; available in PMC: 2018 May 23.
Published in final edited form as: Science. 2017 Dec 14;358(6370):1596–1600. doi: 10.1126/science.aao3642

Fig. 2. RALF4 and RALF19 are pollen tube–expressed and, like BUPS1/2, are required for pollen tube integrity.

Fig. 2

(A and B) Expression pattern of RALF4p::GUS (A) and RALF19p::GUS (B) in flowers. (C and D) Localization of RALF4-GFP (C) and RALF19-GFP (D) in pollen tubes. (E) Mutant alleles of ralf4 and ralf19. Denotations are as described in Fig. 1E. (F and G) Silique length (G) and number of seeds per silique (F) of WT and various mutants. Data are means ± SD (n = 20 siliques); ***P < 0.001 (Student t test). (H) Seed number analysis in reciprocal crosses between WT and homozygous double mutants. ***P < 0.001 (Student t test). (I) Pollen germination assay of ralf4 ralf19 pollen grains. Pictures were collected after 7 hours incubation. (J) Aniline Blue staining of WT and ralf4 ralf19 pollen tubes in WT pistils at 20 hours after pollination. Arrow shows the area where the mutant pollen tubes made contact. Scale bars, 5 mm [(A), (B)], 10 µm [(C), (D)], 50 µm (I), 500 µm (J).