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. 2018 Apr 25;145(8):dev157511. doi: 10.1242/dev.157511

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© 2018. Published by The Company of Biologists Ltd

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Fig. 6. — Characterization of neuroretina cells expressing Cre recombinase in the Cre^Trp1 line. (A) Representative flow cytometry (P5 and young adult) of Cre^Trp1;ROSA^mT/mG. (B) Quantification of total live GFP⁺ and GFP⁻ cells in Cre^Trp1;ROSA^mT/mG retinae. n=24-52 animals/age. (C) RT-qPCR analysis of Hif2a, Vegf and Epo expression in GFP⁺ cells FACS-sorted retina cells from P5 Cre^Trp1;ROSA^mT/mG. n=6-7. ANOVA and Tukey's multiple comparison test: ^§§P<0.01, ^§§§§P<0.0001 vs Cre^Trp1;ROSA^mT/mG. (D) Quantification of GFP⁺ and GFP⁻ cells for each subpopulation analysed by flow cytometry on adult dissociated Cre^Trp1;ROSA^mT/mG retinae. Please refer to Tables S1 and S2 for markers and reference percentages. (E) Histogram comparing the composition of the GFP⁺ subpopulation with the composition of the whole retina of adult Cre^Trp1;ROSA^mT/mG mice (values expressed as percentage of the total retina composition). n=5-9. AC, amacrine cells; GC, ganglion cells; HC, horizontal cells; MG, Müller glia; PR, photoreceptors; rBP, rod bipolar cells.