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. 2018 May 22;16:137. doi: 10.1186/s12967-018-1517-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — ALP staining, ALP activity, collagen secretion and ECM mineralization of cell sheets after osteogenic differentiation. ALP staining, intracellular ALP activity, collagen secretion, ECM mineralization and the quantitative colorimetric results of cell sheets after 3 (a) and 7 days (b) of osteogenic induction. Representative macroscopic view is shown in the upper panels and microscopic view (×40) is shown in the lower panels. Data are shown as mean ± SD and analyzed by Student t-test, n = 3; *p < 0.05, **p < 0.01 and ***p < 0.001