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. 2018 May 22;16:137. doi: 10.1186/s12967-018-1517-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Subcutaneous osteogenesis of cell sheets. Representative H&E (×200) and immunohistochemical staining images (×200) of BMP-2, OCN, VEGF and CD31. New bone formation (NB), osteoid (OS), blood vessels (asterisk) are indicated in the images. Black arrows indicate positive staining cells, red arrows indicate positive staining bone matrix