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. 2017 Jun 21;37(3):BSR20170058. doi: 10.1042/BSR20170058

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© 2017 The Author(s).

This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).

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Results of FACS analysis demonstrated intensification of autophagy process (showed as mean fluorescence intensity – MFI) only at the lowest drug concentration. Application of higher drug doses resulted in silencing of this process and increasing percentage of necrotic cells detected by means of the PI accumulation. (a) The sample results of FACS analysis (G116 culture, 10% adh) based on autophagic vacuoles labelling and PI staining for control cells and the cells treated with increasing tamoxifen concentration. (b) The results reflecting autophagy intensity following the administration of the lowest dose of tamoxifen in particular glioblastoma cultures. Data are expressed as AAF calculated according to the equation: . ↓ - the intensity of autophagy below the level detected in control. (c) Fluorescence microscopy images of control and drug-treated cells confirming the staining capability of autophagy dye (green detection reagent) used for FACS analysis.

Inline graphic — Results of FACS analysis demonstrated intensification of autophagy process (showed as mean fluorescence intensity – MFI) only at the lowest drug concentration. Application of higher drug doses resulted in silencing of this process and increasing percentage of necrotic cells detected by means of the PI accumulation. (a) The sample results of FACS analysis (G116 culture, 10% adh) based on autophagic vacuoles labelling and PI staining for control cells and the cells treated with increasing tamoxifen concentration. (b) The results reflecting autophagy intensity following the administration of the lowest dose of tamoxifen in particular glioblastoma cultures. Data are expressed as AAF calculated according to the equation: . ↓ - the intensity of autophagy below the level detected in control. (c) Fluorescence microscopy images of control and drug-treated cells confirming the staining capability of autophagy dye (green detection reagent) used for FACS analysis.