Figure 4.

Overexpression of Sirtuin 3 (SIRT3) decreases myofibroblast differentiation potential mediated by transforming growth factor beta 1 (TGFβ1). (A–C) Real-time quantitative reverse-transcriptase polymerase chain reaction analysis from normal human lung fibroblasts (NHLF), transfected with adenovirus overexpressing SIRT3, treated with or without TGFβ1, to evaluate transcriptional changes in fibrotic markers collagen type I (COL1), α-smooth muscle actin (α-SMA), and plasminogen activator inhibitor-1 (PAI1). (D) Representative Western blot for PAI1, α-SMA, and SIRT3 expression in NHLF overexpressing SIRT3. β-Actin was used as a loading control. *p < .05, **p < .01, ***p < .005.