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CD73 deficiency promoted microglial M1 polarization and inhibited microglial M2 polarization in BV2 cells, while CD73 overexpression had an opposite effect. After siRNA or pcDNA3.1 transfection for 24 h, BV2 cells were challenged with LPS or IL-4 for another 8 h to activate M1 or M2 activation. a–d Representative two-photon excitation images of IHF acquired using confocal microscope in siRNA-CD73 or pcDNA3.1-CD73 group BV2 cells activated by LPS or IL-4
