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. 2018 May 23;9(4-5):157–171. doi: 10.3727/000000001783992560

Figure 6.

Figure 6

TβR-II protein expression in total lysate, cytosol, and membrane fractions from human breast cell lines. Aliquots (10 μg) of total cell lysate and fractionated protein were analyzed by SDS-PAGE electrophoresis, transferred to PVDF membranes, blocked with 5% nonfat dry milk in PBS-T, and incubated with TβR-II antibody. Bound antibodies were detected by incubation with peroxidase-conjugated antirabbit immunoglobulin and enhanced chemiluminscent autoradiography. A 70-kDa band corresponding to the size expected for TβR-II protein was observed in all cell lines in total lysate and the cytosolic fraction. The membrane fractions demonstrate the clear presence of TβR-II protein in the Mv1Lu positive control, the MCF-10F nontumorigenic, and the tumorigenic MDA-MB231 cell lines. The other cell lines have little or no TβR-II protein present in the membrane fraction.