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. 2018 May 23;9(4-5):195–201. doi: 10.3727/000000001783992605

Figure 1.

Figure 1

Induction of Hsp27 is associated with inhibition of cytochrome c-mediated activation of caspase-3. (A) Cells were maintained at 37°C or heat shocked at 42°C to induce Hsp27. After recovery cells were either further heat shocked at 44°C to induce apoptosis (T) or retained as controls (C). Equal amounts of protein were subjected to SDS-PAGE followed by Western blot analysis with specific antibodies against Hsp27 and PARP. (B) Cytosols prepared from control and thermotolerant cells were measured for DEVDase activity after the addition of cytochrome c/dATP.