Figure 7.

Aberrant expression of neuronal migration genes in adult TTR-HNF-3β transgenic cerebellum. In situ hybridization of wild-type (left panels) and transgenic (right panels) adult cerebellum with ³³P-labeled antisense RNA probes made to neuronal cell migration cDNAs, which are exclusively expressed during cerebellar morphogenesis. Shown are the bright field microscopy (A, B, E, F) and the dark field illumination depicting the hybridization signals (C, D, G, H). In situ hybridization of wild-type or transgenic cerebellum with antisense RNA probe made from the integrin α5 subunit cDNA (C, D) and the ErbB4 receptor cDNA (G, H). The integrin α5 subunit is transiently expressed in radial glial cells in developing cerebella, suggesting an involvement in glial-guided neuronal migration β2), and the ErbB4 receptor cDNA is transiently expressed in Bergmann glial cells during granule cell migration and is the ligand for granule cell neuregulin (58).