Figure 8.

Continued expression of reelin and rcm in neonatal TTR-HNF-3β transgenic cerebellum. In situ hybridization of wild-type (left panels) and transgenic (right panels) P4 cerebellum with ³³P-labeled antisense RNA probes made to various cerebellar marker cDNAs and hybridization signals are shown by dark field illumination (C–H). (A, B) Bright field micrographs of wild-type (A) and transgenic (B) P4 mouse cerebellum demonstrating lack of foliation in transgenic cerebellum stained with hematoxylin/eosin. In situ hybridization of wild-type or transgenic cerebellum with antisense RNA probe made from the Math-1 cDNA (C, D), which labels the external granule cell layer (2,7); the rostral cerebellar malformation (Rcm) cDNA (E, F), which labels both the granule and Purkinje cells (1); and Reelin cDNA (G, H), which labels the external and internal granule cell layers (50,63).