Table 1.

Results of HIV Reservoir Assessment

Time Pointa	Tissue	Assay
		ddPCR Polb	ddPCR Gagc	No. of Cells Assayed by ddPCR	ISH DNAd	ISH RNAe
Pretransplant
–60	PBMCs	211.26	146.11	74 600	-	-
–20	PBMCs	496.43	140.89	112 000	-	-
–8	PBMCs	260.10	152.33	193 000	-	-
	Rectum	71.75	35.80	53 800	2.58 × 105	0
Post-transplant
+22	Rectum	0	0	16 100	2.60 × 104	0
+35	PBMCs	0	0	135 000	-	-
+49	PBMCs	0	0	70 000	-	-
+73 (autopsy)	Brain	0	0	22 600	7.35 × 102	0
	Lung	0	22.57	101 000	1.57 × 104	0
	Lymph node	0	0	85 900	1.06 × 105	0
	Duodenum	0	0	233 000	0	0
	Ileum	0	0	84 600	0	0
	Colon	0	0	166 000	4.16 × 103	0
	Stomach	ND	ND		0	0

Abbreviations: ddPCR, droplet digital polymerase chain reaction; ISH, in situ hybridization; ND, not done; PBMC, peripheral blood mononuclear cell.

^aDays in relationship to transplant (date of transplant = day 0).

^bDigital droplet polymerase chain reaction (PCR) using joPol primer; results reported in DNA copy per 10⁶ cells.

^cDigital droplet PCR using skGag primer; results reported in DNA copy per 10⁶ cells.

^dCopies of HIV DNA per gram of tissue; assay performed on tissue only.

^eCopies of HIV RNA per gram of tissue; assay performed on tissue only.