Fig. 4. (A–D) CLSM images showing the ROS generation of TPETP in MDA-MB-231 cells after different treatments using 2′,7′-dichlorofluorescin diacetate (DCF-DA, 2 μM) as an indicator. The green fluorescence is from DCF-DA (Ex: 488 nm, Em: 505–525 nm). (A–D) share the same scale bar of 50 μm. (E–H) Cell apoptosis imaging after different treatments with light irradiation using annexin V-Cy5 (1 μM) as indicator. The power density was 0.10 W cm–2. Green fluorescence (Rho, Ex: 488 nm; Em: 505–525 nm); red fluorescence (Cy5, Ex: 633 nm, Em: >650 nm). (E–H) share the same scale bar of 20 μm.
